ABSTRACT
Objective To investigate the optimal centrifugation conditions for preparation of rat and mouse platelet rich plasma (PRP) by single centrifugation.Methods Arterial blood of rats and mice by femoral artery cannulation and cardiac puncture were obtained respectively, anticoagulation with 14％CPDA-1, while white blood cells in the blood were filtered out.Then the blood was divided into sterile EP tubes, while PRP was prepared by centrifugation in different conditions (the centrifugal force was 300×g-600×g, and the centrifugal time was 4-12min).The number of blood cells of the anticoagulant whole blood, the leukocyte-depleted blood sample and PRP were counted by hematology analyzer, and platelet recovery rates were compared between different methods.Results The platelet recovery rate was highest when the blood samples of rats and mice were centrifuged at 400×g and 300×g for 8min respectively.Conclusion It is a key to prepare PRP by single centrifugation that selecting the appropriate centrifugal force and time and reaching a critical state before the formation of the buffy coat.
ABSTRACT
Objective To investigate the incidence of altitude reaction among people who rapidly ascend to highaltitude and the health status of experimental emergency blood donors after ascending to high -altitude areas above 4600meters.Methods The vital signs of 185 subjects were analyzed according to GJB1098-1991 and questionaires were filledout.Then the incidence of altitude reaction in different -altitude areas and the change in health status before and after blooddonation at high-altitude were evaluated.Results There was no significant difference in the incidence of altitude reactionin areas of different altitude.However, the incidence of moderate and severe altitude stress increased significantly .Thesimilar altitude reaction incidence despire different means of transportation (74.40% by railway and 70.00% by air) suggestedthat the mode of transportation had little effect.And our results showed good health status among emergency blood donorsbefore and after donation.Conclusion Emergency blood collection is feasible at high altitude (more than 4600 meter high).
ABSTRACT
Objective Human immunoglobulin combinatorial library was generated by using phage surface display expression system, and phage antibodies (Fabs) to platelet were screened.Methods:PBMC were separated from a patient who Were transfusion refractory to platelet. mRNA was isolated and cDNA was synthesized by reverse transcriptase.The immunoglobulin heavy chain Fd and the light chain ? genes were amplified by half nested PCR and the PCR products were cloned into the expression vector pCome3 respectively.The immunoglobulin combinatorial library was constructed and screened by 3 rounds of affinity selection.Results Human Immunoglobulin Combinatorial Library was successfully constructed.The specific phage antibodies were highly enriched after 3 rounds of biopanning selection against platelet membrane proteins.Conclusion The antibody library and human monoclonal antibodies to platelet may be useful as molecular tools to study the anti platelet drugs, platelet related diseases, epitope of human platelet antigens.